Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Primary antibodies used for WBs and IHC.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Western Blot, Recombinant

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Demographics for LSCC and control patients. Cohort A: n = 15 LSCC and n = 7 controls (SRP) for proteome analysis; Cohort B: n = 75 LSCC and n = 54 controls (SRP) for validation by ELISA and WBs; in some cases, not all data were available. Mean values and standard deviation were calculated using Microsoft Excel (Version 2404, Redmond, WA, USA), p -values were determined using GraphPad Prism (Version 9.3.1, La Jolla, CA, USA).

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Control, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Multiplexed array results showing the fold changes between LSCC and control patients. The FC of IGFBP7 and ANXA1 for different stages of LSCC are displayed (see Supplementary Data S1 for results of all proteins of the proteomic analysis). Tumor samples included n = 4 patients with T1N0 LSCC, n = 2 patients with T2N0 LSCC, n = 3 patients with T4 (N0) LSCC, and n = 6 patients with T4 (N+) LSCC. The control group consisted of n = 7 samples from patients undergoing SRP. FCs were calculated by dividing the mean values of the tumor groups by the mean value of the control group. Data analysis was provided by Raybiotech. Median normalization of the data was performed using the analysis tool provided by Raybiotech. Known exosome markers that were also analyzed with the antibody array were used as references for further normalization, via geNorm.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Control, Ab Array

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: ELISA results for IGFBP7 and ANXA1 on whole serum and exosomes. ( a ) IGFBP7 on whole serum comparing control patients and different stages of LSCC. For all tumor sizes, IGFBP7 showed a significant overexpression in LSCC compared to controls. ( b ) IGFBP7 on exosomes isolated from serum comparing control patients to different stages of LSCC. IGFPB7 showed significant overexpression for all tumor sizes compared to controls. ( c ) ANXA1 on whole serum comparing control patients and different stages of LSCC. For all tumor sizes, except T4, ANXA1 showed a significant downregulation in LSCC compared to controls. ( d ) ANXA1 on exosomes isolated from serum comparing control patients to different stages of LSCC. For all tumor sizes, except T2, ANXA showed significant overexpression compared to controls. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, not significant. For the calculation of ELISA results and the creation of graphics, GraphPad Prism Version 9.3.1 (GraphPad Software, La Jolla, CA, USA) was used. Differences between the groups were determined using the Kruskal-Wallis-test. p -values < 0.05 were considered as statistically significant.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Over Expression, Isolation, Software

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve to distinguish LSCC (T1 and T2) patients from healthy subjects by IGFBP7 ( a ) and ANXA1 ( b ). ( a ) ROC curve for IGFBP7: area under the ROC curve (AUC) 0.9128; standard error a 0.03235; 95% confidence interval b 0.8494 to 0.9762; significance level p < 0.0001. ( b ) ROC curve for ANXA1: area under the ROC curve (AUC) 0.7148; 0standard error a 0.0623; 95% confidence interval b 0.5927 to 0.8369; significance level p = 0.0023. ROC curves for IGFBP7 and ANXA1 were calculated using GraphPad Prism Version 9.3.1 (GraphPad Software, La Jolla, CA, USA).

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Software

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Immunohistochemistry of LSCC and control tissue for IGFBP7 and ANXA1. Tissue sections were photographed using a BZ-X810 microscope with BZ-X800 viewer and analyzer software (Keyence, Germany, Neu-Isenburg). Images were taken at 20× and 40× magnification. Scale bars were included. Contrast and brightness were adjusted.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Immunohistochemistry, Control, Microscopy, Software

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Western blots on tissue lysates of different stages of LSCC (T1–T4) and controls for IGFBP7 and ANXA1. In total n = 8 LSCC and n = 6 control tissue lysate samples were analyzed for IGFBP7 and ANXA1. WBs signals were imaged using ChemStudio PLUS (Analytik Jena, Jena, Germany). Band intensity was quantified using VisionWorks version 8.2 (Analytik Jena, Jena, Germany). Results were normalized to GAPDH. Semiquantitative densitometric analysis of the band patterns and normalization to GAPDH yielded the FC value of 3.81 for IGFBP7 and 0.84 for Annexin A1. IGFBP7: All stages of LSCC showed a stronger staining in comparison to controls. ANXA1: Western blots on tissue lysates of different stages of LSCC and controls for ANXA1. The band pattern of ANAX1 showed hardly any differences between the tumor and the control samples. GAPDH: Reference protein for WB analysis showing a consistent band intensity for all samples.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Western Blot, Control, Staining, Comparison

Journal: Cancers

Article Title: Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

doi: 10.3390/cancers16112028

Figure Lengend Snippet: Quantitative, densitometric analysis of Western blot results are presented in . The absolute signal intensities of the respective samples were normalized to the absolute signal intensities of GAPDH. In total n = 8 LSCC and n = 6 control tissue lysate samples were analyzed for IGFBP7 and ANXA1. Signals were imaged using ChemStudio PLUS (Analytik Jena, Jena, Germany). Densitometric analysis was performed by band intensity quantification via the image processing software VisionWorks version 8.2 (Analytik Jena, Jena, Germany). Results were normalized to GAPDH. Abbreviations: au, arbitrary units. *** p < 0.001; ns, not significant.

Article Snippet: The Human Annexin A1 PicoKine ELISA No. EK1745 (Boster Biological Technology, Pleasanton, CA, USA) was used.

Techniques: Western Blot, Control, Software